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表面活性剂是由两亲性分子组成的化合物，含有亲水和疏水分子，能够分隔不同的物理界面。生物表面活性剂是天然产品，以可持续方式生产，对环境无害。单鼠李糖脂(mRL)是一种糖脂类生物表面活性剂，通过PBAD-SD启动子控制源自铜绿假单胞菌ZS1的rhlAB基因表达，利用恶臭假单胞菌KT2440异源生产mRL，在0.02%阿拉伯糖诱导下可以产生单鼠李糖脂的最高生产水平1.73 g·L-1。乳化分析结果表明：原油作为不混溶化合物时，mRL达到100%的E24乳化指数的最低浓度为1g·L-1。因此，mRL可以用于油污染的环境修复。恶臭假单胞菌KT2440中的可调控PBAD-SD启动子是生产mRL等生物表面活性剂的有效系统。
Abstract：Surfactants are compounds composed of amphiphilic molecules, containing hydrophilic and hydrophobic molecules that separate different physical interfaces. Biosurfactants are natural products, produced in a sustainable manner and are not harmful to the environment. Mono-rhamnolipid(mRL) is a glycolipid biosurfactant. m RL was heterologously produced using Pseudomonas putida KT2440 by controlling the expression of the rhlAB gene derived from Pseudomonas aeruginosa ZS1 via the PBAD-SDpromoter, and the highest production level of mono-rhamnolipid of 1.73 g·L-1could be produced under 0.02% arabinose induction. Emulsification analyses showed that crude oils as an immiscible compound, the lowest concentration of m RL to achieve 100%of the E24 emulsification index was 1 g·L-1. m RL can therefore be used for environmental remediation of oil contamination. The tunable PBAD-SDpromoter in Pseudomonas putida KT2440 is an effective system for the production of biosurfactants such as m RL.
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基本信息:
中图分类号:X505;X172
引用信息:
[1]李雪莲,童秀芳,张浩杨,等.恶臭假单胞菌KT2440中基于P_(BAD-SD)启动子的单鼠李糖脂表达系统构建[J].浙江海洋大学学报(自然科学版),2025,44(05):449-455.
基金信息:
浙江省自然科学基金项目(LQ24C010003)
2025-09-15
2025-09-15